DIGE labeling kit and DIGE Trial Pack for 2-D DIGE

DIGE (2-D Fluorescence Difference Gel Electrophoresis) is the most cited technique for accurate quantitation of differences in protein expression using 2-D electrophoresis. Two new products are offered: a 2 nmol CyDye™ DIGE Fluor minimal dye labeling kit and a 2 nmol DIGE Trial Pack. The new labeling kit offers a convenient means of trying DIGE for the first time or for running a small scouting experiment. It contains 2 nmol of each of the CyDye DIGE Fluor minimal dyes Cy™2, Cy3, and Cy5. The quantity in the package is sufficient to run five gels (10 protein samples plus internal standards). The DIGE Trial Pack contains 2 nmol of all three CyDye DIGE Fluor minimal dyes and a free, 1-month trial license of DeCyder™ 2-D Differential Analysis Software, which is the dedicated image analysis software for the Ettan™ DIGE system.

The minimal dye labeling kit employs the minimal DIGE labeling approach, which involves labeling of the lysine animo acid. The CyDye minimal dyes are size- and charge-matched, which allows coseparation of all three protein samples (sample 1, sample 2, and pooled sample) in the same gel. The minimal labeling approach is the most commonly used DIGE protocol and is suitable for all sample types.

Key to the Ettan DIGE approach is a fluorescent imager such as the Typhoon™ Imager or Ettan™ DIGE Imager. No post-stain of the gels is needed.

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