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An automated three-step purification of the VEGFR2 kinase domain using ÄKTAexplorer (from Life Science News, issue 6)

A histidine-tagged VEGFR2 kinase domain was expressed in baculovirus infected Trichoplusia ni cells and purified using a three-column step procedure (metal chelate affinity, anion exchange, gel ...

An efficient and rapid protein purification and on-column cleavage strategy using GSTrap Columns

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Automated tag removal using ÄKTAxpress (Life Science News 18, p.6-7)

ÄKTAxpress™ chromatography system, an integrated solution for high-throughput purification of His- and GST-tagged proteins, features methods for automated on-column removal of affinity tags. This ...

High-throughput purification of His- and GST-tagged proteins (from Life Science News Issue 17, August 2004)

ÄKTAxpress™ chromatography system is an integrated solution for high-throughput purification of His- and GST-tagged proteins. The system combines hardware, software and specially designed media to ...

Overcoming protein instability problems during fusion protein cleavage

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Purification of a protein tagged with (His)6 at its N-terminus, C-terminus, and both N- and C-termini using different metal ions

Proteins can be labelled with histidine residues at either their N or C terminus. However, the position of the tag may affect protein behavior and, ultimately, its binding to metal ions. To ...

Two-step, automated purification of GST-hippocalcin using GSTrap 4B and HiLoad 16/60 Superdex 200 pg columns on ÄKTAxpress chromatography system (from Discovery Matters Issue 4, June 2006)

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