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About Unattended Protein Purification
The use of recombinant proteins has increased greatly over recent years. The incorporation of fusion tags simplifies the purification process and can also increase yield due to increased solubility of the expressed proteins. Histidine, Strep II, MBP and GST, the most widely used tags for recombinant proteins, allow for protocol automation and rapid purification.
Purification challenges
To obtain the highest protein purity, purification protocols must include multiple steps. However, running many samples simultaneously through multi-step protocols is a tedious and time consuming process using traditional chromatography systems. Additionally, many downstream applications require the removal of the fusion tags, which increases the complexity of the protocol. Completely automating this process allows the user to concentrate on other important tasks.
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Solutions
ÄKTAxpress™ is the first fully integrated automated platform for unattended multi-step purification of tagged proteins and antibodies. ÄKTAxpress is a modular platform that combines hardware, UNICORN™ software, columns and media. Depending on system configuration, ÄKTAxpress means you can:
- Run up to 4 different samples on one system
- Get up to 50 mg of up to >95% pure target proteins without manual intervention
- Use method wizards for creating and using pre-optimized protocols, encompassing on-column tag cleavage
Read more about ÄKTAxpress for tagged protein and ÄKTAxpress MAb for antibody purification
Read more about high throughput protein purification in the documents listed on the left in the navigation bar.
If you wish to be contacted by our local representative about ÄKTAxpress, please fill in this form |
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